The present research work was carried out using a simple RP-HPLC method for simultaneous estimation of zidovudine, lamivudine and nevirapine in bulk and pharmaceutical formulation. The separation was carried out by Phenomenox C18 (4.6 ×250mm, 5µ ID) used as a stationary phase and MeCN: MeOH and 0.1% of DEA (pH 3.0 was adjusted with 10% OPA) used as a mobile phase in the ratio of 30:15:55 at a 0.9ml^-1 flow rate and the peak detection was carried out 245nm (isobestic point). Overall run time was 10.0 min and the retention time was 2.49, 3.98, 7.01 min for zidovudine, lamivudine and nevirapine respectively. The proposed method was validated as per the ICH guidelines and found to be specific, linear, selective and precise. This method can be applied on bulk and commercially available individual and combined pharmaceutical dosage forms.

The  term  ageing  is  a  ubiquitous  biological  phenomenon  that  leads  to  advancing  and deleterious  changes  in  organisms. For  centuries  mankind  has  worked  tirelessly  with  the  goal  of prolonging or if possible, reversing this phenomenon. In recent times, owing to the development of biotechnology and modern therapeutics, there are many promising approaches towards prolonging ageing. One of them involves the usage of stem cell transplantation to depress the detrimental health effects of ageing. Studies showed that aged patients suffering from cardio-vascular diseases have gotten over their ailments upon receiving a single infusion of stem cells from donors who are in their pink of health. Klotho, a gene which was initially discerned as a presumptive anti-ageing gene in mice, has been found to extend the viability of the animal. It has been shown to produce composite phenotypes that resemble early ageing syndromes when there is a defect. Another approach involves Telomeres, an element of chromosomes, which play an important part in ageing as they are repetitive DNA sequences. Resveratrol an antioxidant polyphenols isolated from red wine has been found to possess anti-ageing properties.

Diabetes is a major concern among the diabetic patients due to the constant checking of glucose, planning of meals, hope for eating sugary content meals, daily monitoring of insulin levels. Stem cell plays an important role in treating patients identified with diabetes mellitus. Various researches are conducted on the ability of embryonic stem cells of humans to differentiate into islet cells for defining developmental stages and transcription factors in this process of therapy. However, clinical uses of human embryonic stem cells for treatment are minimal due to formation of teratoma. Diabetes mellitus is a growing health distress of the developing and developed countries beyond the globe. This disease is responsible for the 5% deaths around the world every year. Stem cells are unique to its function of differentiation to various types of specialized cells. The function and properties of both adult stem cells and embryonic stem cells was studied in great detail by the scientists for production of insulin secreting cells. The different type of stem cells includes induced pluripotent stem cells (iPSCs), embryonic stem cells (ESCs) and adult stem cells, which have proven to play an important role in treating diabetes with certain restrictions. This article reviews on the details of advancements, recent research and progress made in the field of stem cell research for treating diabetes, understanding the molecular mechanism of stem cells. This review also deals with the molecular in sights associated with stem cells which regenerate into pancreas, mainly its role in diabetes and also the practical hurdles associated with it.

In this study, to develop a RP-HPLC method for the simultaneous estimation of Simvastatin, Aspirin and Clopidogrel in pharmaceutical combined dosage form. There are many trial and error method performed among these phenomenex ® Gemini C18 column (150 x 4.6mm i.d., 5µm) used as a stationary phase and Acetonitrile: Methanol: 0.05% of Triethylamine (55/15/30 v/v/v), used as a mobile phase and the pH of the aqueous phase adjusted at to 3.5 with 10% ortho phosphoric acid at a 1.0ml/min of flow rate. The peak measurement was performed by UV-detector at λmax of 240nm based on the peak area with linear calibration curves established at concentration of 2-10µg/ml for aspirin, clopidogrel and Simvastatin (where R₂> 0.999 for all three drugs) respectively. The overall runtime was achieved within 10 minutes. The method was validated according to International Conference on Harmonisation (ICH) guidelines to confirm specificity, linearity, accuracy and precision. The proposed validated method was successfully applied for the analysis of bulk and combined marketed dosage forms.

Hemidesmus indicus, a plant in the family Apocynaceae, is used in traditional medicine to treat a wide range of conditions. In this study, an ethanolic extract of the plant's bark was used to see if it had any anti-oxidant properties. The antimicrobial activity of the ethanolic extract was tested against the standard ofloxacin using the disc diffusion method. The DPPH method was used to compare the antioxidant activity of the ethanolic extract to that of a standard ascorbic acid. Even in higher amounts, the extract works against ascorbic acid.